Composite

Part:BBa_K1969014:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-12)


Nano-lantern(cAMP-1.6) regulated by CUP1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 64
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1669
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 64
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 947
    Illegal BsaI.rc site found at 1531
    Illegal SapI.rc site found at 1732


Design Notes

As the CUP1 promoter is a promoter of low expression, and the insertion site at yeast genome has a terminator of its own, we don't cloned any terminator downstream the coding sequence.


Source

It's a combination of two basic parts.

References